ORGANISATION NAMEUniversité Côte d'Azur (UCA)
RESEARCH FIELDNatural sciences
CAREER STAGEFirst Stage Researcher (R1) (Up to the point of PhD)
Vessel Formation in Development and Disease Group
Institut de Biologie Valrose, iBV
CNRS UMR7277 – Inserm U1091
Université Nice Sophia Antipolis
06108 Cedex 2
An ANR funded PhD position is available in the Vessel Formation in
Development and Disease group at the iBV
The role of p16-dependent cellular senescence in healthy aging
Description : Cellular senescence attracts attention as a key player contributing to organismal aging. The
accumulation of senescent cells is dramatically increased with aging, however their precise contribution to
aging-related phenotypes remains largely unclear. In collaboration with the team of D. Bulavin we showed
p16-dependent senescent cells are required for healthy aging. We used different novel inducible mouse
lines to characterise the role of p16 expressing cells in different organs. Currently, we focussed mainly on
liver. The project aims at identifying the cell repertoire linked to aging-induced senescence and to
investigate the impact of senescent cells on liver functions and to understand molecular pathways
modulated by senescence. For these purposes we will use p16-Cre and p16-Cre-ERT2 mice crossed either
with Rosa26-mTmG reporter or Rosa26-DTA ablator mice. The animals will be investigated by histological
and immunohistological methods and RNA sequencing will be performed at different ages. This project will
help to understand the molecular mechanism responsible for aging-induced activation of senescence and
hopefully identify potential molecular targets to manipulate senescence through reprogramming and/or
selective elimination of subsets of senescent cells.
Required Skills : The working language is English.
Experience in molecular biology, cellular biology and/or mouse genetics would be a
Motivation to work with mouse models and team orientation are required. Animal
experimentation training is part of the project.
Grosse, L, Wagner, N, Emelyanov, A, Molina, C, Lacas-Gervais, S, Wagner, KD et al.. Defined p16High Senescent Cell Types Are
Indispensable for Mouse Healthspan. Cell Metab. 2020:. doi: 10.1016/j.cmet.2020.05.002. PubMed PMID:32485135 .
Wagner, KD, Du, S, Martin, L, Leccia, N, Michiels, JF, Wagner, N et al.. Vascular PPARβ/δ Promotes Tumor Angiogenesis and
Progression. Cells. 2019;8 (12):. doi: 10.3390/cells8121623. PubMed PMID:31842402 PubMed Central PMC6952835.
Wagner, KD, El Maï, M, Ladomery, M, Belali, T, Leccia, N, Michiels, JF et al.. Altered VEGF Splicing Isoform Balance in Tumor
Endothelium Involves Activation of Splicing Factors Srpk1 and Srsf1 by the Wilms' Tumor Suppressor Wt1. Cells. 2019;8 (1):. doi:
10.3390/cells8010041. PubMed PMID:30641926 PubMed Central PMC6356959.
Wagner, KD, Ying, Y, Leong, W, Jiang, J, Hu, X, Chen, Y et al.. The differential spatiotemporal expression pattern of shelterin
genes throughout lifespan. Aging (Albany NY). 2017;9 (4):1219-1232. doi: 10.18632/aging.101223. PubMed PMID:28437249
PubMed Central PMC5425123.
Wagner, KD, Cherfils-Vicini, J, Hosen, N, Hohenstein, P, Gilson, E, Hastie, ND et al.. The Wilms' tumour suppressor Wt1 is a major
regulator of tumour angiogenesis and progression. Nat Commun. 2014;5 :5852. doi: 10.1038/ncomms6852. PubMed
El Maï, M, Wagner, KD, Michiels, JF, Ambrosetti, D, Borderie, A, Destree, S et al.. The Telomeric Protein TRF2 Regulates
Angiogenesis by Binding and Activating the PDGFRβ Promoter. Cell Rep. 2014;9 (3):1047-60. doi: 10.1016/j.celrep.2014.09.038.
PubMed PMID:25437559 .
Contacts: Kay-Dietrich Wagner – firstname.lastname@example.org
Nicole Wagner – email@example.com
The responsibility for the funding offers published on this website, including the funding description, lies entirely with the publishing institutions. The application is handled uniquely by the employer, who is also fully responsible for the recruitment and selection processes.